Regulatory Control of Ergosterol Biosynthetic Gene Expression in the Yeast Saccharomyces Cerevisiae
نویسنده
چکیده
Candida albicans, closely related to the model yeast Saccharomyces cerevisiae, is considered to be the most important opportunistic fungal pathogen in human. Azole is a class of antifungal drugs commonly used for current treatment of fungal infections. It mechanically inhibits cytochrome P-450 lanosterol demethylase, the ERG11 gene product and thus blocks ergosterol biosynthesis. In S. cerevisiae, the regulator of drug sensitivity2 (Rds2) is a zinc cluster protein whose deletion results in increased sensitivity to the antifungal drug ketoconazole. When the carbon source is shifted from glucose to alternative carbon sources such as ethanol or lactate, promoter targeting is dramatically altered. Interestingly, Rds2 is found to bind to the promoter of ergosterol biosynthetic gene, including ERG11. In this study, we further examined expression of ERG11 in this model yeast during glucose-lactate shift, via qRT-PCR analysis. We found that the expression of ERG11 gene is minimally affected by the removal of RDS2 in lactate. Thus, the results indicated that Rds2 plays a partial role in mediating the expression of ergosterol biosynthetic ERG11 gene in response to the lactate shift.
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